ACTA AGRONOMICA VOLUME 35. (A MTA AGRÁRTUDOMÁNYI KÖZLEMÉNYEI, 1986)

1986 / 1-2. szám - R. NEHÉZ-S. FAZEKAS-I. ÓVÁRY-V. SZÉKESSY-HERMANN: Myosin preparations from the meristematic tissue of fresh sprouts of vine (Vitis vinifera L., cv. cardinal)

Acta Âgronomica Hungarica, 35 (1—2), pp. 3 — 10 (1986) MYOSIN PREPARATIONS FROM THE MERISTEMATIC TISSUE OF FRESH SPROUTS OF VINE (VITIS VINIFERA L., CV. CARDINAL) R. NEHÉZ1, S. FAZEKAS2, I. ÓVÁRY3 and V. SZÉKESSY-HERMANN2 • CEREAL RESEARCH INSTITUTE, SZEGED, ' 2nd INSTITUTE OF BIOCHEMISTRY AND » PSYCHIATRIC CLINIC, SEMMELWEIS UNIVERSITY MEDICAL SCHOOL, BUDAPEST, HUNGARY (Received: 30 January 1983) KCl-myosin is prepared from the meristematic tissue of vine sprouts. The yield of gel-filtered myosin from 100 g fresh sprouts is about 9.5 mg. This myosin contains 20-30 mol P/mol myosin. With the use of a phosphorylating mixture the phosphate content of the myosin can be increased to 100-150 mol P, depending on the phos­phorylating conditions. The P content of the myosin reaches its maximum value at about 1 mM ATP, within an incubation time of 2 minutes. After basic hydrolysis, P-Arg, P-Lys and two P-His-s (as well as derivatives of traces of 2-3 minor compounds) are found in the control myosin, while the phos­phorylated samples contain a further 3 compounds, together with peak No. 9 as the presumed most labile P-containing derivative. Introduction Actomyosin plays a central role in the basic phenomena of movement in animal organisms. Actomyosin is also found in low grade organisms, e.g. amoebas and slime moulds (WOOLEY 1972, CLARKE and SPUDICH 1974). It is presumed from the evidence of other investigations (see the survey by WAGNER 1979) that actomyosin may also be ubiquitous throughout the plant kingdom. Myosin was previously isolated from a monocotyledonous plant species, maize (Zea mays L.; hybrid SZE DC 384) (NEHÉZ et al. 1985). Myosin prep­arations and their properties are now reported from a dicotyledonous plant species, vine (Vitis vinifera L., cv. Cardinal). About 5-12 mg gel-filtered myosin were obtained from 100 g minced vine sprouts, depending on the meristematic cell content of the sprouts. The rate of phosphorylation, the total phosphate content and the basic amino acid content were also studied. Evidence is given of the "active phos­phate content of myosin", which may play an important role in cytokinetic movements and transport processes, e.g. in the phloem. Material and methods The cv. Cardinal was selected, since the numerous shoots of this variety grow very vigorously in a short period, during the first half of June, and this variety therefore requires very intensive green work. 1* Acta Agronomica Hung. 35, 1986 Akadémiai Kiadó, Budapest

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