Fogorvosi szemle, 2006 (99. évfolyam, 1-6. szám)
2006-04-01 / 2. szám
FOGORVOSI SZEMLE ■ 99. évf. 2. sz. 2006. PDLSCs could be used in regenerative processes in the near future. Under similar circumstances DPSCs yielded a dentinpulp like structure. Thus, DPSCs can be used to investigate the development and regeneration of dental tissues. Strikingly, SHEDs were able to differentiate to nerve- and bone-like tissues in vivo. Based on these results, SHEDs offer a novel way to isolate multipotent adult stem cells, which can be differentiated to various cell lineages. We were able to establish stabilized cell cultures from human periodontal ligament and pulp tissue. In these cultures clonogenic cells were present expressing stem cell markers. Current data from literature and our own results suggest that dental stem cells could be used to regenerate periodontal and dental tissues. Furthermore, dental stem cells can be easily isolated without ethical problems, which limit the clinical application of embryonic stem cells today. DR. B. MOLNÁR,1'2 DR. GY. KÖVESI,1 DR. ZS. DARVAS2 1 Semmelweis University, Department of Periodontology; 2 Semmelweis University, Department of Genetics, Cell- and Immune Biology THE ROLE OF HISTAMINE AND TELOMERASE IN THE MALIGNANT TRANSFORMATION OF ORAL LEUKOPLAKIA Leukoplakia is the most frequent oral precancerous lesion. Homogenous, non-homogenous and erythroleukoplakia have a different risk of malignant transformation. We investigated the role of histamine and telomerase in the transformation process. Presence of histamine-producing histidine decarboxylase (HDC) was detected in tissue samples of leukoplakia by immunohistochemistry, RT-PCR and Western-blot. Histamine levels of tissue samples were measured by HPLC and expression levels of histamine receptors by Real-Time PCR and Westernblot. Telomerase activity was assessed by PCR and ELISA, using telomerspecific primers. Elevated levels of histamine and HDC expression were observed in clinical types with higher risk of malignant transformation. Expression of H2 receptor was found to be significantly higher than the expression of other histamine receptors. Relative telomerase activity (RTA) was different in the clinical types of leukoplakia. Our results suggest that histamine could contribute in the malignisation process by suppressing cytotoxic immune response via H2 receptors. Telomerase activity is essential in immortalisation of cells, RTA levels could be used to predict the risk of malignisation. DR. A. MUKICS, DR. V. TÓTH University of Pécs, School of Dentistry INCREASING THE EFFECTIVENESS OF PERIODONTAL MAINTENANCE THERAPY BY CORSODYL OR HYDROGEN-PEROXIDE GEL APPLYING IN NIGHT GUARD SPLINTS Introduction: The aim of the supportive therapy is to maintain long-term benefical periodontal therapeutic effects. The maintenance of high level oral hygiene requires a highly motivated patient. In cases of decrased patient collaboration or originally lower level of oral hygiene, the use of local chemical plaque control can still have an adjuvant effect. The chemical agents, the patient complience, the costs of the treatment and the time span of the treatment without side effects are all subjects that need to be considered. Materials and methods: In the present study the changes in plaque index and gingival index were evaluated in nonsmoking patients, who were enrolled into periodontal supportive therapy after scaling and root planing (SRP) treatment. Patients were divided in three groups of ten. In Group I SRP was followed with no adjuvant therapy. In Group II and III 5% Corsodyl and 3% hydrogen-peroxide gel respectively was given in a custom made foil-based plastic night guard splint for 30 minutes a day for 21 consecutive days. Results: In Group II and III the plaque index and gingival index were found to be significantly decreased after 3 weeks. Approximately half of the patients using the Corsodyl gel were complaining of oral discomfort. Discussion: In conclusion, the antiseptic agents applied in foil-based plastic night guard splint could be used effectively in gingivitis and plaque control during the short term supportive therapy. The use of 3% hydrogen-peroxide containing gel had no side effects and it’s whitening effect had positive influence on patients’ complience. DR. ZS. NÉMETH TitánDent Private Dental Clinic, Budapest FEATURES OF THE IMPLANT NECK AND THE SUCCESS RATE OF SINUS FLOOR ELEVATION It has been proofed by the high success rate, that the sinus floor elevation is a safe way to broaden the indication field of dental implants in the daily practice. On the one hand, there is the dynamically increasing number of successfully performed sinus elevation during the last 2-3 years. On the other hand, though, the majority of the well-known, popular implant systems are the fruits of successful innovation 10-15 years back. At the time of innovation, sinus floor elevation did not draw as much attention as it does today. The author has the impression that it is almost “by chance” that the given features make the particular implant system suitable or less suitable for sinus floor elevation. The available bone to gain primary stability is usually limited; especially when the sinus floor elevation and the implant insertion is performed in one session. In a situation with thin, compromised bone availability, the shape and surface of the implant neck is determining to achieve stability. Favourable shape, favourable surface means more stability, higher success rate and consequently widening implant indication. Considering the determining features gaining implant stability during sinus floor elevation, the author evaluate different implant neck types, with special respect to BEGO Semados implant system.