Acta Biochimica et Biophysica 11. (1976)
1976 / 4. szám - Kerner, J.-Sándor, A.-Alkonyi, I.: The Effect of Denervation on Carnitine Metabolism in Rat Skeletal Muscle
Kerner et al. : Effect of Denervation on Carnitine Metabolism 241 Table 1 Activity of CPT and CAT and total concentration of carnitine in the innervated soie us and EDL muscles of the rat Denervation caused a striking drop in carnitine concentration in the soleus (Fig. 1). In the white EDL the decrease of the amount of carnitine was less pronounced following a transient increase, and this difference persisted throughout the experiment (Fig. 1). The impairment of active carnitine uptake from the serum by muscles is unlikely to be due to energy deficiency, at least in the early stage of denervation, when both the ATP and creatine phosphate levels are normal CAT CPT Carnitine U/g wet U/mg NCP U/g wet U/mg NCP nmole/g I nmole/mg tissue ±S.E. X 103±S.E. tissue± S.E. X 10я ± S.E. W<± SEUe J NCP±S.E. Soleus* 4.5+0.21 27.7+1.6 1.62+0.05 8.45+0.41 870+11.2 4.53+0.07 (10) (10) (10) (10) (10) ! (10) EDL* 3.7+0.15 18.0+1.23 1.02 + 0.02 5.06+0.2 976+33.9 4.95+0.1 * The differences between the corresponding data of the two muscles are significant (P < 0.05) Figures in parentheses represent the number of animals Fig. 1. Change of muscle carnitine content in the rat soleus (O--------O) and EDL (X--------X) as a function of time after denervation. The concentrations are expressed as nmole/mg NCP and the ratios of concentrations of denervated/control muscles have been plotted. Each point represents the mean from five animals l* Acta Biochimica et Biophysica Academiae Scientiarum Hungaricae 11, 1976