Acta Biochimica et Biophysica 12. (1977)
1977 / 3. szám - Sándor, A.-Kerner, J.-Alkonyi, I.: Effect of Biguanide (Buformin) on Carnitine Polmitoyltransferase Activity
Sándor et al. : Effect of Bigiianide on Carnitine Palmitoyltransferase 219 Fig. 2. The effect of buformin on the reaction (— ) palmitoylcarnitine + CoASH ^ palmitoyl- CoA + L-camitine from right to left. The incubation mixture contained 2 mM L-camitine, 40 /<M dithiobispyridine, 0.1 M Tris (pH 7.5), palmitoyl-CoA as indicated and approximately 1.5 — 2 mU enzyme in a total volume of 1.0 ml at 25 °C. The reaction was followed continuously. o — o no addition; •—• 2.5 mM buformin; x —x calculated rate of a reac]£ tion with thefenzyme saturated with L-carnitine (correction factor, 1 +--------—----L-carnittne ------------------------ (mM) ' palmitoyl-CoA Fig. 3. The effect of buformin on the reaction (—) palmitoylcarnitine + CoASH ^2 palmitoyl-CoA + L-carnitine from left to right. The incubation mixture contained 166 /лМ (—) palmitoyl-(3H)carnitine (specific activity 800 cpm/nmol), 500 /лМ Co A, 2 mM dithioerythritol, 0.1 M Tris (pH 7.5) in a total volume of 0.6 ml at 30 °C. The figure is representative of five experiments. A; 7 mU enzyme, B; 3 mU enzyme, o — o no addition, x—x 2.5 mM buformin Acta Biochimica et Biophysica Academiae Scientiarum Hungaricae 12, 1977