Acta Veterinaria 14. (1964)

1. szám - SIMONYI ERZSÉBET - BOGNÁR KÁROLY - KUCSERA GYÖRGY - REGŐS JÁNOS: Comparative studies on the potency of different crystal-violet swine-fever vaccine batches

4* COMPARATIVE STUDIES ON THE POTENCY OF DIFFERENT CRYSTAL-VIOLET SWINE-FEVER VACCINE BATCHES By Elisabeth Simonyi, K. Bognár, G. Kucsera and J. Regős State Institute for the Control of Veterinary Serobacteriological Products (Director: Elisabeth Simonyi), Budapest (Received January 4, 1963) Crystal violet swine fever vaccine has been applied with more or less success all over the world. The actual importance of its use as well as its advantages and disadvantages have been a subject of discussion both in this country and abroad. A number of authors have reported on the safety and potency tests of crystal violet vaccines. As experimental animal the swine was used in every case; Cole and Henley (1946), Doyle and Wright (1947), Nakamura and Sugimura (1953), Geiger and Poppe (1953), Drager (1955) and Pascu et al. (1961) evaluated the efficiency of the material on the basis of a single vaccination, while Fuchs (1955) and Pehl and Schulze (1956) vaccinated the test animals twice. The dose to be used for challenge, is also under discussion; the amount of swine fever virus administered by the different authors varies between 1000 and 1 million LD. In the course of the safety and potency tests on the crystal violet swine fever vaccine routinely performed in this institute, several considerable obser­vations have been made during the recent years. The tests were performed on pigs by vaccinating on 1 occasion and using a dose of 1 million LD of swine fever virus for challenge. As a matter of course the above vaccine tests generally yield good approximative information, but similarly to other biological methods, the reproducibility of the results is rather poor. The experimental errors involved are mainly due to differences in the individual or hereditary susceptibility of the animals as well as to changes in the experimental conditions. More uniform results can be obtained by increasing the number of test animals and by parallel testing a preparation regarded as standard. We could not afford increasing the number of test animals, but the present examinations were performed throughout with the same virus material prepared and regularly controlled in this institute and stored at —20° C for several months; the test animals were selected from the same breed and several batches of vaccine were examined in parallel experiments. According to our observations made during the recent years, the above examinations and the comparison of the results of repeated tests are sufficient for the

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