Acta Biologica 40. (1989)
1-2. szám - F. Liszt–K. Jobst: Luminol dependent chemiluminescence of quartz and zymosan stimulated neutrophils
Acta Biologica Hungarica 40(1-2), pp. 121—126 (1989) LIJMINOL DEPENDENT CHEMILUMINESCENCE OF QUARTZ AND ZYMOSAN STIMULATED NEUTROPHILS F. LISZT and K. JOBST Department of Clinical Chemistry, University Medical School of Pécs, Hungary (Received 1988-09—13; revised 1988—10—18) The effect of the opsonization by zymosan and quartz particles on the chemiluminescence was investigated on human neutrophil granulocyts. Opsonization of zymosan enhanced the chemiluminescence response, while opsonized quartz inhibited the chemiluminescence reaction. Calcium ionophore A 23187 treatment did not influence the chemiluminescence of quartz but the light signal in the presence of quartz decreased rapidly. In parallel experiments the protein pattern of zymosan treated neutrophils was investigated by high resolution two-dimensional polyacrylamide gel electrophoresis. Keywords: Neutrophil granulocyte — Quartz — Zymosan —Ionophore A 23187 Introduction The chemiluminescence (CL) of luminol has been widely used to detect the generation of reactive oxygen metabolites by various phagocytes since 1972 /1/. It is accepted as a method for evaluating the intensity of the respiratory burst. The luminol or lucigenin dependent CL of polymorphonuclear leukocytes (PMNL) and macrophages have been shown to be triggered by various inorganic dusts (quartz, carbon, asbestos etc.) or organic components e.g. zymosan /3, 4, 8, 9/. The present study was aimed at to investigate the effect of opsonization of zymosan and quartz particles on the CL in human neutrophils. In Send offprint requests to: Dr. K. lobst, Department of Clinical Chemistry, University, Medical School of Pécs, H—7643 Pécs, Ifjúság u. 13, Hungary Akadémiai Kiadó, Budapest