Acta Microbiologica 31. (1984)
2. szám - Mándi, Y.–Bakay, M.–Béládi, I.: Effect of Interferon on Antibody-Dependent Cellular Cytotoxicity (ADCC) in Chickens
4 Acta Microhiologica Hungarica 31(2), pp. 127—133 (1984) EFFECT OF INTERFERON ON ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY (ADCC) IN CHICKENS Yvette Mándi, Márta Bakay and Ilona Béládi Institute of Microbiology, University Medical School, Szeged (Received February 18, 1983) The influence of chicken leukocyte interferon (IFN) on the antibody-dependent cellular cytotoxicity (ADCC) of peripheral blood mononuclear cells of chicken has been investigated in vivo and in vitro. The cytotoxic effect was studied on sheep red blood cells (SRBC) coated with chicken anti-SRBC serum. Cytotoxicity was estimated using a 51Cr release assay system. A single intravenous injection of crude IFN led to an enhanced ADCC 6 to 12 h after the IFN injection, whereas by 24 h the activity had decreased. The ability of IFN to augment the cytotoxicity was not lost after phagocytic cell depletion. In vitro, the ÁDCC was boosted within 1—18 h by IFN, in a dose-dependent manner. We assume that the augmenting agent in vitro is IFN itself, as purified IFN had a similar activity, but mock IFN and trypsin-treated IFN showed no enhancing effect. In connection with the various effects of interferon (IFN) on immune responses, much attention has been focussed on the ability of IFN to augment the cytotoxic reactivity of natural killer (NK) cells and also to increase the antibody-dependent cellular cytotoxicity (ADCC). The ADCC reaction measures the cytotoxicity of unsensitized effector cells against IgG-coaled target cells different in origin. While the effects of IFN on mouse and human NK cells [1, 2] and the ADCC [3, 4] have been well studied, no data are available about these effects on chicken leukocyte IFN. We have recently shown [5] that human adenovirus enhances the ADCC in chickens, and we assumed that IFN induced by the virus was responsible for the effect. Earlier studies from this laboratory [6] proved that adenoviruses induce IFN in chickens, and it thus seemed of interest to examine the effect of chicken IFN on the ADCC. In this paper we describe the results of experiments dealing with the effects of chicken IFN on the ADCC in vivo and in vitro in a xenogeneic test system, using antibody-coated sheep red blood cells (SRBC) as target cells. We present evidence that chicken leukocyte IFN enhances the ADCC both in vitro and in vivo. Materials and methods Chickens. Six-week-old birds of the White Leghorn strain (Hyline Poultry Farm, Johnston, Iowa) were used for experiments in vivo. To study the in vitro effect of IFN, effector cells were obtained from 8-12-week old birds. Yvette Mándi, Marta Bakay, Ilona Béládi Institute of Mierobiology, University Medical School Dóm tér 10, H-6720 Szeged, Hungary Acta Microhiologica Hungarica 31, 1984