Acta Physiologica 71. (1988)
1. szám - Abstracts of the lectures and posters delivered and demonstrated at the XVI. MEMBRANE TRANSPORT SYMPOSIUM, Sümeg, May 12-16, 1986, Hungary - I. LECTURES - Gabriella Bátory-G. Görög-T. Laskay-F. Péterfy-G. Petrányi: Effect of monoclonal antibodies to lymphocyte surface structure on lymphocyte functions
Abstracts ated by IgG-Fc, they were used for mapping of the functionally active parts of the IgG molecule, as well as in studying the mechanism of Fc-dependent effector functions. Effect of monoclonal antibodies to lymphocyte surface structure on lymphocyte functions GABRIELLA BÁTORY, G. GÖRÖG, T. LASKAY, F. PÉTERFY, G. PETRÁNYI BLOOD PRODUCTS LABORATORY EGIS PHARMACEUTICAL WORKS AND NATIONAL INSTITUTE OF HAEMATOLOGY AND BLOOD TRANSFUSION, BUDAPEST, HUNGARY The Second International Workshop on Leucocyte Differentiation provided the possibility to investigate MoAbs to well defined cell surface structures in multiple number for each specificity. The effect of monoclonal antibodies to T cell antigens were investigated on the following lymphocyte functions: — killer activity: natural lectin dependent and antibody dependent cellular cytotoxicity; — cell proliferation: proliferative effect on resting peripheral mononuclear cells, effect on mitogen and antigen stimulation of lymphocytes and on IL2 dependent cell proliferation; •— release of cell products of mitogen stimulated lymphocytes: Interleukin-2 (IL2), Interferon (IFN), and Immunoglobulin (IgG and IgM) production. The most important concluions drawn from our results are as follows: -— the complexity of a given function can be characterized by the number of structures through which the function in question can he influenced; — the effectivity spectra of different antibodies specific for a given structure allow to suppose the involvement of the structure in different activation mechanisms; -— the selective inhibition by MoAb of lymphocyte activation phenomena suggest that different activation mechanisms rather than a single chain of sequences are responsible for their appearance; — the intracellular mechanism of lymphocyte activation pathways may be elucidated by the use of such MoAbs in biochemical studies of intracellular events.