ACTA ALIMENTARIA VOL. 6 (A QUARTERLY OF THE COMMITTEE ON FOOD SCIENCE OF THE HUNGARIAN ACADEMY OF SCIENCES, 1977)
1977 / 1. sz. - KOZMA-KOVÁCS, E.-KEVEI-PICHLER, E.-LENDVAI, I.: Determination of the capsaicin content in raw green paprika
2 KOZMA-KOVÁCS et a].: CAPSAICIN DETERMINATION IN GREEN PAPRIKA they are not suitable for determination of low capsaicin contents. Extracts containing 50-150 pg capsaicin were applied to the layer and these correspond to a capsaicin content of 500-1000 mg per 100 g sample. Literature on quantitative thin-layer chromatography of ground paprika is listed in Table 1. Table 1 Thin-layer chromatographic methods for the quantitative determination oj capsaicin in ground paprika The first method applying thin-layer chromatography was developed by HEUSSER (1964). The procedure is lengthy because capsaicin is separated after running twice in chloroform in order to eliminate lipoids. The chromatograms could be evaluated visually and then capsaicin could be eluted to determine its quantity by spectrophotometry. However, this procedure requires an extract containing 100-200 pg capsaicin. The method of SPANYÁR and BLAZOVICH (1969) is exceptional because it is suitable for the analysis of ground paprika samples containing above or below 10 mg capsaicin in 100 g. The extraction procedure is different for samples of low and high capsaicin content. An extract containing 0.2-0.6 pg capsaicin is needed for determination. Based on the method of SPANYÁR and BLAZOVICH (1969), Bios and DUDEN (1971) as well as ANDRE and MILE (1972) developed thin-layer chroma- Authors Adsorbent Developing agent Detecting reagent Sensitivity of the detecting reagent HEUSSER (1964) Kieselgel HF251 1. Twice in chloroform (to eliminate lipoids) 2. Chloroformmethanol-acetic acid (95 : 1 : 5) a) 0.4% 2,6 dibromoquinone chloroimide dissolved in methanol b) Ammonia atmosphere 1 I»g SPANYÁR and BLAZOVICH (1969) Kieselgel G Chloroform-methanol (99 : 1) 15% FeCl3; 0.5% KJIVKCN).], 1 : 1 aqueous solution 0.1 pg Rios and DUDEN (1971) Kieselgel G Chloroform-ethyl acetate-isopropanol (33 : 9 : 3) 281 nm Zeiss-Chromatogram-Spectralphotometer 2 pg ANDRÉ and MILE (1972) Kieselgel G Diethyl ether a) 0.2% dichloroquinone chloroimide dissolved in methanol b) 0.05 N NaOH, 50% solution in methanol 0.2 pg Ac/a Alimentaria в, 1977