Acta Morphologica 24. (1976)

1-2. szám - Pathologia - Nagy Judit–Pár A.–Bajtai G.–Ambrus Mária–Deák G.: Membraneous Glomerulonephritis Induced by HB8 (Australia) Antigen-Antibody Complexes

GLOMERULONEPHRITIS INDUCED BY IIB„ 131 visible by clotted blood is seen and covered with sharp edges. The adjacent area is not scarred. The small intestine contains some melenic substance. Grave sclerotic lesions, partly atheromatous and partly ulcerative, are visible in the abdominal aorta; in the upper and lower thirds there is tightly adher­ing clot that does not show any sign of organization. The lumen from the orifice of the coeliac to that of the mesenteric artery and even the orifices them­selves are covered with loosely adhering fresh clot which constricts to about one fourth the origin of the right renal artery. Light microscopic examinations. In the liver, widened periportal areas and pseudolobular transformation is seen, with chronic, lobular infiltration in the connective tissue that penetrates between the hepatic lobes separating the cell groups. In the kidneys, diffuse thickening of all glomeruli could be observed, without notable cell proliferation (Fig. 1). Immunofluorescence studies. Cryostate sections were washed in phos­phate buffered salt solution (PBS) and incubated with fluorescein isothio­cyanate (FITC)-conjugated anti-human IgG, IgM, IgA, C3, and fibrinogen (Hyland, Costa Mesa, Calif.). Indirect immunofluorescent method was applied for the detection of HBS antigen. After washing in PBS the sections were incubated with anti-human IIBS produced in rabbit (Behringwerke A. G., Marburg), then repeated washing in PBS was followed by incubation with FITC-conjugated anti-rabbit sheep 7S immunoglobulin (Progressive Labora­tories). Finally, the sections were mounted under a coverslip with PBS- glycerol and examined under a Zeiss fluorescent microscope. The examinations were completed with the parallel study of the extinc­ Fig. 1. Diffuse thickening of glomerular basement membrane without notable cell proliferation (Pas, X 400) 9* Acta Morphologien Academiae Scientiarum Hungaricae 24, 1976

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