Acta Medica 46. (1989)
1989 / 1. szám - IMMUNOLOGY - Cs. Balázs-E. Kiss-N. R. Farid: Effect of lithium on chemiluminescence of polymorphonuclear granulocytes and mononuclear cells of peripheral blood
34 BALÁZS, CS. et al. Fig. 1 Effect of lithium on chemiluminescence (mean +S.E.M.) of mononuclear cells, induced by PHA-P (10 /jg/ml). Luminol-dependent chemiluminescence activity of mononuclear cells induced by PHA-P. The ordinate shows the light output in mV, the abscissa the speed of paper (1.0 mm/min). These continuously-registered curves represent the time-dependent process of chemiluminescence. The significance was calculated from automatically registered (in each 10 s) integrated values from ten experiments. This experiment indicates that lithium exhibited a transient increase in chemiluminescence followed by a significant decrease in presence of 2.0 mM (P <0.01) and 5.0 mM (P<0.001) of lithium respectively) (Fig. 1). In contrast, when mononuclear cells were activated through Fc-receptors by opsonized Zymosan the chemiluminescence activity decreased already at 1.0 mM. At higher concentrations an obvious inhibition was found (at 1.0-2.0 mM P < 0.05, at 5.0 mM P < 0.01) (Fig. 2). It is noteworthy that the biphasic early activation was observed only mononuclear cells were activated via the mitogen receptor. The respiratory burst of PMN by opsonized Zymosan and PHA the chemiluminescence was slightly different from that of mononuclear cells. The mitogen-induced activation was monophasic and dose-dependent in the presence of increasing concentrations of lithium. At 1.0 mM of lithium a minimal inhibition was observed in chemiluminescence, but 2.0 mM and 5.0 mM concentrations resulted in significant decrease in photon emission (P <0.05 and P <0.001) (Fig. 3). The opsonized Zymosan induced Fc-receptor-dependent respiratory burst